superblocktm buffer (Thermo Fisher)
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superblocktm buffer
Superblocktm Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tween+20+pbs/Tween+20+blocking+buffer%2C+1%25+in+PBS/pm42271423-86-12-14
Average 95 stars, based on 1 article reviews
Superblocktm Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tween+20+pbs/Tween+20+blocking+buffer%2C+1%25+in+PBS/pm42271423-86-12-14
Average 95 stars, based on 1 article reviews
superblocktm buffer - by Bioz Stars,
2026-09
95/100 stars
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Reverse Transcription:Article Title: Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in situ sequencing (mudRapp-seq). Article Snippet: .. For padlock probing carried out on a cDNA target, cells ere washed with 1 × PBS containing 0.05% Staining:Article Title: Influenza A virus induces PI4P production at the endoplasmic reticulum in an ATG16L1-dependent manner to promote the egress of viral ribonucleoproteins Article Snippet: Cells were then incubated overnight at 4 °C with primary antibodies directed against NP and GFP to amplify the Sec61ß-mEmerald signal (Abcam 13970, 1:500) in staining buffer (PBS supplemented with 0.2% Triton X-100, 0.125% fish gelatin, 5% Normal Donkey serum and 3% Normal Goat Serum). .. Cells were then washed three times in 0.05% Article Title: Influenza A virus induces PI4P production at the endoplasmic reticulum in an ATG16L1-dependent manner to promote the egress of viral ribonucleoproteins Article Snippet: Cells were then incubated overnight at 4 °C with primary antibodies directed against RAB11 (Invitrogen 71-5300, 1:100), CLIMP63 (R&D systems AF7355, 1:500), ATG16L1 (MBL PM040, 1:200), the HA tag (Invitrogen 26183, 1:100), or the influenza NP (BioRad MCA 400 or Invitrogen PA5-32242, 1:1000). .. Cells were then washed three times in 0,05% Incubation:Article Title: Investigation of the prevalence of Cryptosporidium parvum infection in hemodialysis patients with recombinant CP2 and CP23 antigens Article Snippet: Cryptosporidium parvum is an intracellular protozoan parasite that poses significant health risks, particularly in immunocompromised (Crawford and Kol 2021).. As a causative agent of cryptosporidiosis, C. parvum primarily infects the gastrointestinal tract, leading to symptoms such as watery diarrhea, abdominal pain, nausea, and vomiting (Harp 2003).. In healthy individuals, cryptosporidiosis typically manifests as a self-limiting illness; however, in immunocompromised populations such as dialysis patients, the infection can lead to severe, prolonged, and potentially lifethreatening complications. Article Title: UBC9 silencing-mediated PPARα desumoylation induces inhibition of cell proliferation by ferroptosis in acute myeloid leukemia Article Snippet: Primary antibodies for PPARα (ab227074, 52 kDa, 1:500, Abcam, UK), SUMO1 (ab32058, 12 kDa, 1:1000, Abcam, UK), SLC7A11 (ab175186, 55 kDa, 1:1000, Abcam, UK), GPX4 (ab125066, 17 kDa, 1:1000, Abcam, UK) and GAPDH (ab8245, 37 kDa, 1:500, Abcam, UK) were utilized to probe the membrane overnight at 4°C. .. The membrane was then washed thrice with Enzyme-linked Immunosorbent Assay:Article Title: Investigation of the prevalence of Cryptosporidium parvum infection in hemodialysis patients with recombinant CP2 and CP23 antigens Article Snippet: Cryptosporidium parvum is an intracellular protozoan parasite that poses significant health risks, particularly in immunocompromised (Crawford and Kol 2021).. As a causative agent of cryptosporidiosis, C. parvum primarily infects the gastrointestinal tract, leading to symptoms such as watery diarrhea, abdominal pain, nausea, and vomiting (Harp 2003).. In healthy individuals, cryptosporidiosis typically manifests as a self-limiting illness; however, in immunocompromised populations such as dialysis patients, the infection can lead to severe, prolonged, and potentially lifethreatening complications. Membrane:Article Title: UBC9 silencing-mediated PPARα desumoylation induces inhibition of cell proliferation by ferroptosis in acute myeloid leukemia Article Snippet: Primary antibodies for PPARα (ab227074, 52 kDa, 1:500, Abcam, UK), SUMO1 (ab32058, 12 kDa, 1:1000, Abcam, UK), SLC7A11 (ab175186, 55 kDa, 1:1000, Abcam, UK), GPX4 (ab125066, 17 kDa, 1:1000, Abcam, UK) and GAPDH (ab8245, 37 kDa, 1:500, Abcam, UK) were utilized to probe the membrane overnight at 4°C. .. The membrane was then washed thrice with Blocking Assay:Article Title: Influenza A virus induces PI4P production at the endoplasmic reticulum in an ATG16L1-dependent manner to promote the egress of viral ribonucleoproteins Article Snippet: Cells were then incubated overnight at 4 °C with primary antibodies directed against RAB11 (Invitrogen 71-5300, 1:100), CLIMP63 (R&D systems AF7355, 1:500), ATG16L1 (MBL PM040, 1:200), the HA tag (Invitrogen 26183, 1:100), or the influenza NP (BioRad MCA 400 or Invitrogen PA5-32242, 1:1000). .. Cells were then washed three times in 0,05% |